Purified Agar is a water soluble colloidal extract obtained from certain species of marine red algae including Gelidium, Pterocladia, and Gracilaria. Impurities, debris, minerals, and pigment are reduced to specific levels during manufacture. The majority of microorganisms cannot digest agar thus making it an excellent solidifying agent. The addition of 13-15 grams/Liter is recommended for the preparation of most dehydrated culture media formulations. If the culture medium has a pH of 6 or less 20 grams/Liter should be used. Semi-solid medium is obtained with agar concentrations of 3-8 grams/Liter. Purified Agar is extensively washed and bleached. This agar should be used where extreme clarity and purity is required. Purified Agar is used as a solidification agent in the preparation of microbiological and tissue culture media, immuno diffusion, and electrophoresis. (Alpha Biosciences # A01-102P-500gm)
Use: Purified Agar is used as a solidification agent in the preparation of microbiological and tissue culture media, immuno diffusion, and electrophoresis.
Description: Purified Agar is a water soluble colloidal extract obtained from certain species of marine red algae including Gelidium, Pterocladia, and Gracilaria. Impurities, debris, minerals, and pigment are reduced to specific levels during manufacture. The majority of microorganisms cannot digest agar thus making it an excellent solidifying agent. The addition of 13-15 grams/Liter is recommended for the preparation of most dehydrated culture media formulations. If the culture medium has a pH of 6 or less 20 grams/Liter should be used. Semi-solid medium is obtained with agar concentrations of 3-8 grams/Liter. Purified Agar is extensively washed and bleached. This agar should be used where extreme clarity and purity is required.
 Typical Physical & Chemical Characteristics:
- Color: White Cream
- Ash: < 1.5%
- Moisture: < 10%
- Gel Strength: 700-1200 g/cm²
- pH in gel: 5.8 - 7.0 (1.5% solution)
- Gel Point: 32.0 - 37.5°C
- Melting Point: 80 - 95°C ± 0.5°C
- Clarity (1.5%): < 100 NP
- Colorimetry (absorbance 430nm): < 0.150
- Resistivity (1%) 25,000 Ohms
- Electroendosmosis (EEO): < 0.450
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original white cream color.
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