(AB) Brain Heart Infusion Agar, 2 Kilograms

Brain Heart Infusion (BHI) Agar has proved to be effective in the cultivation of a wide variety of microorganisms, including many types of pathogens. BHI Agar is currently recommended as a general medium for aerobic bacteria and for the primary recovery of fungi from clinical specimens. BHI Agar, with 10% Sheep Blood can be used to isolate systemic fungi that may grow poorly on nonenriched media. A high recovery rate for Histoplasma capsulatum can be obtained with the addition of horse blood to the medium. Brain Heart Infusion Agar is used for cultivating a wide variety of fastidious and non-fastidious microorganisms, including bacteria, yeasts and fungi. (Alpha Biosciences # B02-112-2Kg)

Use: Brain Heart Infusion Agar is used for cultivating a wide variety of fastidious and non-fastidious microorganisms, including bacteria, yeasts and fungi.

Description: Brain Heart Infusion (BHI) Agar has proved to be effective in the cultivation of a wide variety of microorganisms, including many types of pathogens. BHI Agar is currently recommended as a general medium for aerobic bacteria and for the primary recovery of fungi from clinical specimens. BHI Agar, with 10% Sheep Blood can be used to isolate systemic fungi that may grow poorly on nonenriched media. A high recovery rate for Histoplasma capsulatum can be obtained with the addition of horse blood to the medium.

 Formula* per Liter:

• Brain Heart Infusion from Solids..... 8.0g
• Dextrose ........................................ 2.0g
• Casein Digest Peptone..................16.0g
• Sodium Chloride ........................... 5.0g
• Peptic Digest of Animal Tissue ..... 5.0g
• Disodium Phosphate .....................2.5g
• Agar ...............................................13.5g

 Final pH: 7.4 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 52.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Quality Control Specifications:

1. The powder is homogeneous, free flowing and light beige.

2. Visually the prepared medium is clear and yellow beige

3. Expected cultural response after 18-24 hours at 35.0°C.

Organism                                                                                        Result

Candida albicans ATCC 10231                                                       Growth

Neisseria meningitides ATCC 13090                                              Growth

Streptococcus pneumonia ATCC 6305                                           Growth

Streptococcus pyogenes ATCC 19615                                           Growth

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color