(AB) Brilliant Green Bile Agar 500G

Brilliant Green Bile Agar is used for the rapid detection of coliform bacteria in water and sewage. The APHA recommends the use of this medium as an indication of the extent of contamination. The medium is not recommended for the precise determination of coliforms in water samples. The selective agents in the medium are brilliant green and bile which inhibited Gram positive bacteria. Basic fuchsin and erioglaucine are pH indicators which turn red to purple in response to acid fermentation of lactose. Lactose-fermenting bacteria (E coli) produce deep red colonies. The medium is very sensitive to light and should be used on the day it is prepared. Brilliant Green Bile Agar is used for the detection, isolation and enumeration of coliform bacteria in water, and sewage. (Alpha Biosciences # B02-122-500gm)

Use: Brilliant Green Bile Agar is used for the detection, isolation and enumeration of coliform bacteria in water, and sewage.

Description: Brilliant Green Bile Agar is used for the rapid detection of coliform bacteria in water and sewage. The APHA recommends the use of this medium as an indication of the extent of contamination. The medium is not recommended for the precise determination of coliforms in water samples. The selective agents in the medium are brilliant green and bile which inhibited Gram positive bacteria. Basic fuchsin and erioglaucine are pH indicators which turn red to purple in response to acid fermentation of lactose. Lactose-fermenting bacteria (E coli) produce deep red colonies. The medium is very sensitive to light and should be used on the day it is prepared.

Formula* per Liter:

• Pancreatic Digest of Gelatin .......... 8.25g
• Lactose .................................. 1.9g
• Sodium Sulfite ................................. 0.25g
• Ferric Chloride ................ 0.0295g
• Monopotassium Phosphate ....... 0.0153g
• Erioglaucine .................... 0.0649g
• Basic Fuchsin .............................. 0.0776g
• Oxgall ............................. 0.00295g
• Brilliant Green ....................... 0.0000295g
• Agar .................................... 10.15g

 

Final pH: 6.9 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 20.6 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Quality Control Specifications:

1. The powder is homogeneous, free flowing and purple to beige.
2. Visually the prepared medium is turquoise blue and clear to trace hazy.
3. Expected cultural response after 24-48 hours at 35.0°C.

Organism                                                                                 Result

Enterobacter aerogenes ATCC 13048                      Good growth, Pink colonies.

Escherichia coli ATCC 25922                                   Good Growth, Red colonies.

Salmonella typhimurium ATCC 14028                      Good Growth, Clear colonies.

Staphylococcus aureus ATCC 25923                                     Inhibited

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original purple to beige color.