(AB) Brilliant Green Bile Broth 2%, 500 grams

Brilliant Green 2% Bile Broth is used to confirm the presence of coliforms. Oxbile and Brilliant Green inhibit Gram positive bacteria and many Gram negative bacteria other than coliforms. Lactose is a carbohydrate source. Bacteria that ferment lactose and produce gas are detected. This medium is formulated according to APHA and AOAC specifications. Brilliant Green Bile Broth 2% is used for confirming the presence of coliforms in water, sewage and dairy products. (Alpha Biosciences # B02-118-500gm)

Use: Brilliant Green Bile Broth 2% is used for confirming the presence of coliforms in water, sewage and dairy products.

Description: Brilliant Green 2% Bile Broth is used to confirm the presence of coliforms. Oxbile and Brilliant Green inhibit Gram positive bacteria and many Gram negative bacteria other than coliforms. Lactose is a carbohydrate source. Bacteria that ferment lactose and produce gas are detected. This medium is formulated according to APHA and AOAC specifications.

Formula* per Liter:

• Pancreatic Digest of Gelatin .......... 10.0g
• Lactose ................................ 10.0g
• Oxbile ................................................ 20.0g
• Brilliant Green ................. 0.0133g

Final pH: 7.2 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 40.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Quality Control Specifications:

1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is emerald green and clear.
3. Expected cultural response after 18-48 hours at 35.0°C.

Organism                                                                              Result

Escherichia coli ATCC 25922                                          Growth, Gas (+)

Enterobacter aerogenes ATCC 13048                            Growth, Gas (+)

Enterococcus faecalis ATCC 29212                                Inhibited, Gas (-)

Staphylococcus aureus ATCC 25923                              Inhibited, Gas (-)

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light greenish beige color.