Product Overview
Edel and Kampelmacher noted that food preservation techniques such as heat, desiccation, preservatives, high osmotic pressures or pH changes can cause sub-lethal injury Salmonella microorganisms. Preenrichment with Buffered Peptone Water allows for repair of cell damage by maintaining a high pH over the preenrichment period. The high pH capacity is especially useful for vegetable samples which have a low buffering capacity. Buffered Peptone Water is used for preenriching damaged Salmonella sp. from various food sources to increase recovery. (Alpha Biosciences # B02-121-500gm)
Use: Buffered Peptone Water is used for preenriching damaged Salmonella sp. from various food sources to increase recovery.
Description: Edel and Kampelmacher noted that food preservation techniques such as heat, desiccation, preservatives, high osmotic pressures or pH changes can cause sub-lethal injury Salmonella microorganisms. Preenrichment with Buffered Peptone Water allows for repair of cell damage by maintaining a high pH over the preenrichment period. The high pH capacity is especially useful for vegetable samples which have a low buffering capacity.
Formula* per Liter:
- Pancreatic Digest of Gelatin ... 10.0g
- Sodium Chloride ........................ 5.0g
- Disodium Phosphate .................. 1.5g
- Monopotassium Phosphate ...... 3.5g
Final pH: 7.2 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 20.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.
Quality Control Specifications:
- The powder is homogeneous, free flowing and light beige.
- Visually the prepared medium is clear and pale yellow.
- Expected cultural response after 18-24 hours at 35.0°C.
Organism Result
Escherichia coli ATCC 25922 Growth
Salmonella enteritidis ATCC 13076 Growth
Salmonella typhi ATCC 19430 Growth
Salmonella typhimurium ATCC 14028 Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.