Columbia Blood Agar Base is usually prepared with horse, sheep, or rabbit blood to determine the hemolytic reactions of fastidious pathogenic microorganisms. Columbia Blood Agar Base can be prepared as Blood Agar or Chocolate Agar or used without blood as a general purpose medium. This medium contains a highly nutritious combination of peptones. The addition of starch enhances the recovery of Neisseria, and hemolytic reactions of some streptococci. Columbia Blood Agar Base is used for the cultivation of fastidious and non-fastidious microorganisms with or without the addition of blood. (Alpha Biosciences # C03-111-2Kg)
Use: Columbia Blood Agar Base is used for the cultivation of fastidious and non-fastidious microorganisms with or without the addition of blood.
Description: Columbia Blood Agar Base is usually prepared with horse, sheep, or rabbit blood to determine the hemolytic reactions of fastidious pathogenic microorganisms. Columbia Blood Agar Base can be prepared as Blood Agar or Chocolate Agar or used without blood as a general purpose medium. This medium contains a highly nutritious combination of peptones. The addition of starch enhances the recovery of Neisseria, and hemolytic reactions of some streptococci.
Formula* per Liter:
- Casein Digest Peptone .................. 5.0g
- Corn Starch ................................... 1.0g
- Peptic Digest of Animal Tissue ..... 8.0g
- Sodium Chloride ........................... 5.0g
- Yeast Enriched Peptone.............. 10.0g
- Agar ...............................................14.0g
Final pH: 7.3 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preperation: Mix 43 grams of the medium in a Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes. Prepare 5 to 10% blood agar by adding the appropriate volume of sterile defibrinated blood to melted sterile agar medium, cooled to 45-50°C. Dispense into Petri dishes or tubes
Quality Control Specifications:
- The powder is homogeneous, free flowing and light beige.
- Visually the prepared medium is opaque and cherry red.
- Expected cultural response after 18-24 hours at 35.0°C under 5-10 CO2.
Organism                                                       Result                           Hemolysis
Escherichia coli ATCC 25922                        Growth                                  -
Staphylococcus aureus ATCC 25923            Growth                               Beta
Streptococcus pneumonia ATCC 6305         Growth                               Alpha
Streptococcus pyogenes ATCC 19615         Growth                                Beta
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
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