The solid meat granules in the broth medium provide favorable growth conditions for anaerobic microorganisms due to the reducing action of the sulfhydryl groups of the muscle proteins. The cooked (denatured) protein makes these groups more readily accessible. This medium can be used to differentiate saccharolytic from proteolytic Clostridium sp. Saccharolytic species rapidly form acid and gas without digesting the meat. Proteolytic species break down the meat (blackening) to amino acids and form sulfur compounds (putrid smell). APHA and ACAC recommend Cook Meat Medium for the recovery of Clostridium botulinum from foods. Hulman recommended this medium for stock cultures. Cooked Meat Medium is used for cultivating anaerobic microorganisms and for stock culture maintenance. (Alpha Biosciences # C03-114-500gm)
Use: Cooked Meat Medium is used for cultivating anaerobic microorganisms and for stock culture maintenance.
Description: The solid meat granules in the broth medium provide favorable growth conditions for anaerobic microorganisms due to the reducing action of the sulfhydryl groups of the muscle proteins. The cooked (denatured) protein makes these groups more readily accessible. This medium can be used to differentiate saccharolytic from proteolytic Clostridium sp. Saccharolytic species rapidly form acid and gas without digesting the meat. Proteolytic species break down the meat (blackening) to amino acids and form sulfur compounds (putrid smell). APHA and ACAC recommend Cook Meat Medium for the recovery of Clostridium botulinum from foods. Hulman recommended this medium for stock cultures.
Formula* per Liter
- Beef Heart ....................................... 454g
- Dextrose ......................................... 2g
- Casein Digest Peptone................. 10.0g
- Sodium Chloride ............................ 5g
- Peptic Digest of Animal Tissue ... 10.0g
Final pH: 7.2 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Place 1.25 grams of meat granules into a test tube and add 1.0 mL of purified water. Autoclave at 121°C for 15 minutes.
Quality Control Specifications:
- The medium is dehydrated brown pellets.
- The prepared medium in a test tube appears as medium clear amber supernatant over insoluble granules.
- Expected cultural response after 48-72 hours at 35.0°C.
Organism                                                                                     Result
Clostildium perfringens ATCC 13124Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Clostridium sporogenes ATCC 11437Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Escherichia coli ATCC 25922Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Staphylococcus aureus ATCC 25923Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original brown pellets.
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