Corn Meal Agar is valuable for morphologic differentiation of may yeast-like organisms. It suppresses vegetative growth of many fungi while stimulating sporulation. Corn Meal Agar allows Candida albicans to produce chlamydospores which is one of the best criterion for identification. Kelly and Funigello reported that the addition of 1% Tween 80 enhanced chlamydospore formation. Corn Meal Agar is used for stimulating the production of chlamydospores by most strains of Candida albicans and for cultivating phytopathological fungi. (Alpha Biosciences # C03-115-500gm)
Use: Corn Meal Agar is used for stimulating the production of chlamydospores by most strains of Candida albicans and for cultivating phytopathological fungi.
Description: Corn Meal Agar is valuable for morphologic differentiation of may yeast-like organisms. It suppresses vegetative growth of many fungi while stimulating sporulation. Corn Meal Agar allows Candida albicans to produce chlamydospores which is one of the best criterion for identification. Kelly and Funigello reported that the addition of 1% Tween 80 enhanced chlamydospore formation.
Formula* per Liter:
Corn Meal..................................... 15.0g
Agar ............................................... 2.0g
Final pH: 6.0 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preperation: Mix 17 grams of the medium in a Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes. Cool to 45-50°C then dispense into Petri dishes or tubes.
Quality Control Specifications:
- The powder is homogeneous, free flowing and light yellow.
- Visually the prepared medium is opaque and white.
- Expected cultural response after 40-48 hours at 25.0°C.
Organism                                                            Result                     Chlamydospores
Aspergillus niger ATCC 16404                           Growth                                   -
Candida albicans ATCC 10231                          Growth                                  +
Saccharomyces cerevisiae ATCC 18790           Growth                                   -
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light yellow color.
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