Cystine Tryptic Agar was formulated by Vera . Many workers have used this medium for the cultivation of various fastidious microorganisms such as Neisseria, Streptococcus, Pasteurella, Brucella, Corynebacterium and Vibrio. Anaerobes were also capable of growth in this medium without the addition of anaerobic conditions. Mycoplasma could be cultivated with the addition of 5% yeast extract and rabbit serum. Cystine Tryptic Agar is a semi-solid medium used for the cultivation, maintenance, motility determination and fermentation reactions of bacteria. (Alpha Biosciences # C03-116-500gm)
Use: Cystine Tryptic Agar is a semi-solid medium used for the cultivation, maintenance, motility determination and fermentation reactions of bacteria.
Description: Cystine Tryptic Agar was formulated by Vera . Many workers have used this medium for the cultivation of various fastidious microorganisms such as Neisseria, Streptococcus, Pasteurella, Brucella, Corynebacterium and Vibrio. Anaerobes were also capable of growth in this medium without the addition of anaerobic conditions. Mycoplasma could be cultivated with the addition of 5% yeast extract and rabbit serum.
Formula* per Liter:
- Casein Digest Peptone ................ 20.0g
- L-Cystine ....................................... 0.5g
- Sodium Sulfite .................................. 0.5g
- Sodium Chloride ........................... 5.0g
- Phenol Red................................... 0.017g
- Agar ............................................... 2.5g
Final pH: 7.3 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 28.5 grams of the medium in one Liter of purified water until evenly dispersed. Add desired carbohydrate, heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave for 15 minutes at 115.0°C. DO NOT OVERHEAT Medium.
Quality Control Specifications:
- The powder is homogeneous, free flowing and light beige.
- Visually the prepared medium is red and clear to trace hazy.
- Expected cultural response after 18-24 hours at 35.0°C.
Organism                                                                             Result
Escherichia coli ATCC 25922Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Neisseria gonorrhoeae ATCC 43069Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Neisseria meningitides ATCC 13090Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Streptococcus pneumonia ATCC 6305Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
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