D/E Neutralizing Agar is capable of neutralizing a broad spectrum of antiseptic and disinfectant chemicals including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde and glutaraldehyde. It can determine the bactericidal capability of disinfectants and therefore is well suited for environmental sampling. Agar medium used for the testing and neutralizing of antiseptics and disinfectants. (Alpha Biosciences # D04-112-2Kg)
Use: Agar medium used for the testing and neutralizing of antiseptics and disinfectants.
Description: D/E Neutralizing Agar is capable of neutralizing a broad spectrum of antiseptic and disinfectant chemicals including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde and glutaraldehyde. It can determine the bactericidal capability of disinfectants and therefore is well suited for environmental sampling.
Formula* per Liter:
- Yeast Extract .................................... 2.5g
- Dextrose ...................................... 10.0g
- Casein Digest Peptone................... 5.0g
- Lecithin........................................... 7.0g
- Sodium Thioglycollate ..................... 1.0g
- Sodium Thiosulfate ....................... 6.0g
- Sodium Bisulfite ............................... 2.5g
- Polysorbate 80 .............................. 5.0g
- Bromocresol Purple....................... 0.02g
- Agar ...............................................15.0g
Final pH: 7.6 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 54 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.
Quality Control Specifications:
- The powder is homogeneous, moist, lumpy and bluish grey
- Visually the prepared medium is opaque and lavender.
- Expected cultural response after 24-48 hours at 35.0°C.
Organism                                                                                   Result
Bacillus subtilis ATCC 6633Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Escherichia coli ATCC 25922Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Pseudomonas aeruginosa ATCC 27853Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Salmonella typhimurium ATCC 14028Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light bluish grey color.
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