Dextrose Tryptone Broth is a non-selective medium which can support the growth of a variety of mesophilic and thermophilic microorganisms. It contains the pH indicator bromcresol purple. Inoculated filter membranes are incubated on this medium at 55°C to detect and enumerate thermophilic "flat sour" sporulating microorganisms. Dextrose Tryptone Broth is a broth medium used for the cultivation of "FLAT SOUR" organisms related to the spoilage of food products. (Alpha Biosciences # D04-111-500gm)
Use: Dextrose Tryptone Broth is a broth medium used for the cultivation of "FLAT SOUR" organisms related to the spoilage of food products.
Description: Dextrose Tryptone Broth is a non-selective medium which can support the growth of a variety of mesophilic and thermophilic microorganisms. It contains the pH indicator bromcresol purple. Inoculated filter membranes are incubated on this medium at 55°C to detect and enumerate thermophilic "flat sour" sporulating microorganisms.
Formula* per Liter:
- Tryptone .......................................... 20.0g
- Dextrose ..................................... 10.0g
- Bromcresol Purple ......................... 0.04g
Final pH: 6.7 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 30 grams of the dehydrated medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for minute to dissolve completely. Distribute and autoclave at 121°C for 15 minutes.
Quality Control Specifications:
- The powder is homogeneous, free-flowing and light greenish beige.
- Visually the prepared medium is clear and dark burgendy.
- Expected cultural response after 36-48 hours at 55.0°C.
Organism                                                                        Result
Bacillus coagulans ATCC 7050Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Bacillus stearothermophilus ATCC 7953Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 8.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light greenish beige color.
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