Various workers described the use of sulfhydryl compounds and low concentrations of agar, alone or in combination, to culture anaerobic organisms in a fluid broth under aerobic conditions. Other workers described the use of sodium thioglycollate in these broth media for the neutralization of mercurial preservatives. Sodium thioglycollate aIso lowers the oxidation-reduction potential of the media. Resazurin indicates the status of oxidation or aerobiosis. Dextrose is added to enhance growth. Fluid Thioglycollate Medium with 5% Beef Extract is recommended by Animal and Plant Health Inspection Service, USDA, in the detection of viable bacteria in live vaccines. Thioglycollate Culture Medium with 5% Beef Extract isused for the cultivation of anaerobic, microaerophilic and aerobic microorganisms and for determining the presence of bacteria in normally sterile materials. Formulated according to the USP and AOAC. (Alpha Biosciences # F06-104-500gm)
Use: Thioglycollate Culture Medium with 5% Beef Extract isused for the cultivation of anaerobic, microaerophilic and aerobic microorganisms and for determining the presence of bacteria in normally sterile materials. Formulated according to the USP and AOAC.
Description: Various workers described the use of sulfhydryl compounds and low concentrations of agar, alone or in combination, to culture anaerobic organisms in a fluid broth under aerobic conditions. Other workers described the use of sodium thioglycollate in these broth media for the neutralization of mercurial preservatives. Sodium thioglycollate aIso lowers the oxidation-reduction potential of the media. Resazurin indicates the status of oxidation or aerobiosis. Dextrose is added to enhance growth. Fluid Thioglycollate Medium with 5% Beef Extract is recommended by Animal and Plant Health Inspection Service, USDA, in the detection of viable bacteria in live vaccines.
Formula* per Liter
- Yeast Extract .................................... 5.0g
- Dextrose ........................................ 5.5g
- Casein Digest Peptone................. 15.0g
- Sodium Chloride .......................... 2.5g
- Sodium Thioglycollate ..................... 0.5g
- L-Cystine ....................................... 0.5g
- Resazurin ..................................... 0.001g
- Agar ............................................. 0.75g
- Beef Extract...................................... 5.0g
Final pH: 7.2 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 34.7g of medium in one Liter of purified water until evenly dispersed Heat with repeated stirring and boil for one minute to dissolve completely. Dispense and sterilize in the autoclave at 121.0°C for 15 minutes. After storage of the medium, if 30% of the medium demonstrates oxidation (color change of indicator), reheat the medium once in boiling water or steam bath.
Quality Control Specifications:
- The powders are homogeneous, free flowing and light beige.
- The prepared medium is yellow colored, hazy or clear, with upper 10% or less light to medium red upon standing for those media containing the rezasurin dye.
- Expected cultural response after 24-48 hours at 35.0°C.
Organism                                                                         Result
Bacillus subtilis ATCC 9372Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Bacteroides vulgatus ATCC 8482Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Candida albicans ATCC 10231Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Clostridium sporogenes ATCC 11437Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Micrococcus luteus ATCC 9341Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige.
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