Heart Infusion Broth medium is prepared from an infusion of beef heart. The first media used for the cultivation of bacteria was prepared from meat infusions. Huntoon showed that pathogenic microorganisms could be grown from infusion agar without enrichments. Broth medium recommended for the cultivation of fastidious microorganisms. (Alpha Biosciences # H08-101-500gm)
Use: Broth medium recommended for the cultivation of fastidious microorganisms.
Description: Heart Infusion Broth medium is prepared from an infusion of beef heart. The first media used for the cultivation of bacteria was prepared from meat infusions. Huntoon showed that pathogenic microorganisms could be grown from infusion agar without enrichments.
Formula* per Liter:
- Brain Heart Infusion from Solids..... 2.0g
- Yeast Extract ................................. 3.0g
- Casein Digest Peptone..................10.0g
- Sodium Chloride ........................... 5.0g
- Peptic Digest of Animal Tissue ..... 5.0g
Final pH: 7.4 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 25 grams of the medium in a Liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve. Distribute and autoclave at 121°C for 15 minutes.
Quality Control Specifications:
- The powder is homogeneous, free flowing and light beige.
- Visually the prepared medium is light to medium amber and clear.
- Expected cultural response after 18-48 hours at 35.0°C.
Organism                                                                                     Result
Escherichia coli ATCC 25922Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Staphylococcus aureus ATCC 25922Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Streptococcus pneumonia ATCC 6305Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Streptococcus pyogenes ATCC 19615Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.
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