(AB) LB Broth, 2 Kilograms

The inclusion of casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals and vitamins. Sodium chloride provides essential electrolytes. LB Broth is used in molecular genetic studies. (Alpha Biosciences # L12-101-2Kg)

Use: LB Broth is used in molecular genetic studies.

Description: The inclusion of casein peptone and yeast extract supply essential growth factors, such as nitrogen, carbon, sulfur, minerals and vitamins. Sodium chloride provides essential electrolytes.

Formula* per Liter:

• Casein Digest Peptone .......... 10.0 g
• Yeast Extract ...................... 5.0g
• Sodium Chloride ........................ 5.0g

Final pH: 7.0 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 20.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Quality Control Specifications:

1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is yellow to gold and clear to moderately hazy.
3. Expected cultural response after 18-24 hours at 35.0°C.

Organism                                                                            Result

Escherichia coli ATCC 23724                                              Growth

Escherichia coli ATCC 33694                                              Growth

Escherichia coli ATCC 33849                                              Growth

Escherichia coli ATCC 39403                                              Growth

Escherichia coli ATCC 47014                                              Growth

Escherichia coli ATCC 53868                                              Growth

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.