Product Overview
Use: Broth medium recommended for the detection of coliforms in water, wastewater and foods.
Formula* per Liter:
- Casein Digest Peptone ............. 20.0g
- Potassium Phosphate, Monobasic. 2.75g
- Lactose .......................................... 5.0g
- Potassium Phosphate, Dibasic....... 2.75g
- Sodium Chloride ............................5.0g
- Sodium Lauryl Sulfate ........................ 0.1g
Final pH: 6.8 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 35.6 grams of the medium in one Liter of purified water until evenly dispersed. Prepare double strength broth for evaluating 10 mL samples. Distribute into test tubes containing inverted
Quality Control Specifications:
- The powder is homogeneous, free flowing and light beige.
- Visually the prepared medium is yellow to gold and clear to trace hazy.
- Expected cultural response after 18-24 hours at 35.0°C.
Organism                                                                               Result
Enterobacter aerogenes ATCC 13048Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth, Gas +
Escherichia coli ATCC 25922Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth, Gas +
Proteus mirabilis ATCC 12453Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth, Gas -
Salmonella typhimurium ATCC 14028Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Growth, Gas -
Staphylococcus aureus ATCC 25923Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â No Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.