Product Overview
Use: Malt Extract Agar is used for the isolation, cultivation and maintenance of yeasts and molds.
Description: Malt Extract Agar formulation is similar to Malt Extract Broth with the addition of agar and glycerol. This broth medium was recommended by Thom and Church for the cultivation of fungi. Its low acidity inhibits the growth of most bacteria. The high carbohydrate content enhances the rapid growth of fungi and yeasts.
Formula* per Liter:
- Maltose, Technical .................... 12.75g
- Dextrin ........................................ 2.75g
- Glycerol ......................................... 2.35g
- Peptone ...................................... 0.78g
- Agar ..............................................15.0g
Final pH: 4.6 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 33.6 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes. DO NOT OVERHEAT.
Quality Control Specifications:
- The powder is homogeneous, free flowing and light beige.
- Visually the prepared medium is grayish white and moderately hazy.
- Expected cultural responseafter 40-72 hours at 25-30.0°C.
Organism                                                                                       Result
Aspergillus
Candida albicans ATCC 10231Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Off-white creamy colonies
Saccharomyces cerevisiae ATCC 18790Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Off-white creamy colonies
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.