Product Overview
Use: Malt Extract Broth is used for the cultivation and maintenance of yeasts and molds.
Description: This broth medium was recommended by Thom and Church for the cultivation of fungi. Its low acidity inhibits the growth of most bacteria. The high carbohydrate content enhances the rapid growth of fungi and yeasts.
Formula* per Liter:
- Malt Extract Base .......................... 6.0g
- Dextrose ....................................... 6.0g
- Maltose, Technical ........................ 1.8g
- Yeast Extract ................................ 1.2g
Final pH: 4.7 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 15.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.
Quality Control Specifications:
- The powder is homogeneous, free flowing and light beige.
- Visually the prepared medium is very light amber and clear.
- Expected cultural response after 40-72 hours at 25-30.0°C.
Organism                                                              Result
Aspergillus
Candida albicans ATCC 10231Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Good Growth
Saccharomyces cerevisiae ATCC 18790Â Â Â Â Â Â Â Â Â Â Good Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.