(AB) Motility Test Medium, 2 Kilograms

Motility Test Agar is a modification of the original formula used to detect bacterial motility. The medium is inoculated by stabbing though the center of the prepared medium. Motility is observed macroscopically by observing growth radiating from the original stab. Certain strains demonstrate motility by diffusing throughout the medium while others only show a small amount of diffusion along the lateral line of inoculation. Temperature of incubation can also be a factor in demonstrating motility as certain bacteria such as Yersenia enterocolitica show better motility at 25.0°C. Motility Test Agar is a semisolid medium used for the determination of bacterial motility. (Alpha Biosciences # M13-121-2Kg)

Use: Motility Test Agar is a semisolid medium used for the determination of bacterial motility.

Description: Motility Test Agar is a modification of the original formula used to detect bacterial motility. The medium is inoculated by stabbing though the center of the prepared medium. Motility is observed macroscopically by observing growth radiating from the original stab. Certain strains demonstrate motility by diffusing throughout the medium while others only show a small amount of diffusion along the lateral line of inoculation. Temperature of incubation can also be a factor in demonstrating motility as certain bacteria such as Yersenia enterocolitica show better motility at 25.0°C.

Formula* per Liter:

• Beef Extract ................................. 3.0g
• Sodium Chloride ........................5.0g
• Pancreatic Digest of Gelatin .....10.0g
• Agar ............................................ 4.0g

Final pH: 7.3 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 22.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Quality Control Specifications:

1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is yellow beige and clear to trace hazy.
3. Expected cultural response after 18-24 hours at 25°C.

Organism                                                                          Result

Escherchia coli ATCC 25922                                    Growth, Motility +

Shigella flexneri ATCC 12022                                   Growth, Motility -

Enterococcus faecalis ATCC 29212                          Growth, Motility -

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.