(AB) Peptone Water, 2 Kilograms

Peptone Water is a minimal growth medium which is used for determining carbohydrate fermentation capabilities. A 1% final carbohydrate concentration with phenol red (0.018 g/L) indicator and Durham tube can be used for fermentation studies. After incubation a yellow broth with or without gas bubbles indicates a positive fermentation reaction. For determining the Indole Test place an indole test strip 10 mm above a 24-48 hr. broth culture and reincubate for 5-30 minutes. A violet red color on the test strip indicates a positive reaction. Broth medium used for the detection of indole and carbohydrate fermentation patterns. (Alpha Biosciences # P16-103-2Kg)

Use: Broth medium used for the detection of indole and carbohydrate fermentation patterns.

Description: Peptone Water is a minimal growth medium which is used for determining carbohydrate fermentation capabilities. A 1% final carbohydrate concentration with phenol red (0.018 g/L) indicator and Durham tube can be used for fermentation studies. After incubation a yellow broth with or without gas bubbles indicates a positive fermentation reaction. For determining the Indole Test place an indole test strip 10 mm above a 24-48 hr. broth culture and reincubate for 5-30 minutes. A violet red color on the test strip indicates a positive reaction.

Formula* per Liter:

• Peptone..................................... 10.0 g
• Sodium Chloride ........................ 5.0g

Final pH: 7.2 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 15.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Quality Control Specifications:

1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is gold to amber and clear to trace hazy.
3. Expected cultural response after 18-24 hours at 35.0°C.

Organism                                                                             Result

Escherichia coli ATCC 25922                                              Growth

Salmonella typhimurium ATCC 14028                                  Growth

Staphylococcus aureus ATCC 25923                                   Growth

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.