(AB) Psudeo Iso Agar, 2 Kilograms

Pseudomonas Isolation Agar is a modification of a formula described by King et al. Pseudomonas Isolation Agar was found useful in the isolation of Pseudomonas from pharmaceutical preparations (lotions and creams). The incorporation of lrgasan (R) inhibits most bacteria but allows the growth of Pseudomonas. The formulation also enhances pyocyanin production. Selective media used for the isolation of Pseudomonas. (Alpha Biosciences # P16-114-2Kg)

Use: Selective media used for the isolation of Pseudomonas.

Description: Pseudomonas Isolation Agar is a modification of a formula described by King et al. Pseudomonas Isolation Agar was found useful in the isolation of Pseudomonas from pharmaceutical preparations (lotions and creams). The incorporation of lrgasan (R) inhibits most bacteria but allows the growth of Pseudomonas. The formulation also enhances pyocyanin production.

Formula* per Liter:

• Pancreatic Digest of Gelatin ...... 20.0g
• Magnesium Chloride ..................... 1.4g
• Potassium Sulfate ....................... 10.0g
• Irgasan (R) ................................. 0.025g
• Agar ..............................................13.6g

Final pH: 7.0 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 45 grams of the agar medium and 20 ml of glycerol in 980 ml of purified water. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Quality Control Specifications:

1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is yellow beige and clear to trace hazy.
3. Expected cultural response after 18-48 hours at 35.0°C.

Organism                                                                            Result

Escherichia coli ATCC 25922                                            Inhibited

Proteus mirabilis ATCC 12453                                          Inhibited

Pseudomonas aeruginosa ATCC 10145            Growth, blue-green colonies

Pseudomonas aeruginosa ATCC 27853            Growth, blue-green colonies

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.