(AB) SDA, 2 Kilograms

Sabouraud Dextrose Agar is an agar medium recommended for the isolation, cultivation and identification of non-pathogenic and pathogenic fungi. This medium does not contain antibiotics or inhibitors and can be used for the cultivation of those fungi which are susceptible to antibiotics. Agar medium used for the isolation, identification and cultivation of fungi. (Alpha Biosciences # S19-100-2Kg)

Use: Agar medium used for the isolation, identification and cultivation of fungi.

Description: Sabouraud Dextrose Agar is an agar medium recommended for the isolation, cultivation and identification of non-pathogenic and pathogenic fungi. This medium does not contain antibiotics or inhibitors and can be used for the cultivation of those fungi which are susceptible to antibiotics.

Formula* per Liter:

• Casein Digest Peptone................. 10.0g
• Dextrose .......................................40.0g
• Agar ............................................. 15.0g

Final pH: 5.6 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 65.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Quality Control Specifications:

1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is clear to trace hazy and light amber in color.
3. Expected cultural response after 24-72 hours at 25-30.0°C.

Organism                                                                    Result

Aspergillus niger ATCC 16404                      Woolly and black mycelium

Candida albicans ATCC 10231                     Off-white creamy colonies

Saccharomyces cerevisiae ATCC 18790       Off-white creamy colonies

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.