Product Overview
Use: Agar medium used for the isolation, identification and cultivation of fungi.
Description: Sabouraud Dextrose Agar is an agar medium recommended for the isolation, cultivation and identification of non-pathogenic and pathogenic fungi. This medium contains cholamphenicol, a broad spectrum antibiotic, which is inhibitory to a wide range of Gram negative and Gram positive bacteria.
Formula* per Liter:
- Casein Digest Peptone................. 5.0g
- Dextrose .......................................40.0g
- Peptic Digest of Animal Tissue...10.0g
- Agar ............................................. 12.0g
- Chloramphenicol......................... 0.05g
Final pH: 5.6 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance
Preparation: Mix 65.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.
Quality Control Specifications:
- The powder is homogeneous, free flowing and light beige.
- Visually the prepared medium is clear to trace hazy and light amber in color.
- Expected cultural response after 24-72 hours at 25-30.0°C.
Organism                                                                         Result
Aspergillus
Candida albicans ATCC 10231Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Off-white creamy colonies
Trychophyton mentagrophytes ATCC 9533Â Â Â Â Â Â Â Â Â Â Off white to tan & powdery
Escherichia coli ATCC 25922Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â Â No Growth
Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.