Trypto Soy Broth

Use: Trypto Soy Broth is a general purpose broth medium used for the cultivation of fastidious and non-fastidious microorganisms. it is prepared according to the formula listed as Soybean-Casein Digest Medium in the United States Pharmacopeia XXI (1985).

Description: The inclusion of casein peptone and soy peptone to the formula enhances the growth of many fastidious microorganisms without the addition of blood or serum. It can support the growth of a wide variety of microorganisms including aerobes, anaerobes and fungi. Because of its nutritional capabilities it can be used as an enrichment broth and as a blood culture medium.

Formula* per Liter:
Casein Digest Peptone ................ 17.0g
Papaic Digest of Soybean Meal .... 3.0g
Disodium Phosphate ....................... 2.5g
Dextrose ........................................ 2.5g
Sodium Chloride ........................... 5.0g

Final pH: 7.3 ± 0.2 at 25.0°C
* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 30.0 grams of the medium in one Liter of purified water until evenly dispersed. Heat with repeated stirring and boil for one minute to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes.

Quality Control Specifications:
1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is very light amber and clear.
3. Expected cultural response after 18-24 hours at 35.0°C

Organism
Bacillus subtilis ATCC 6633
Bacteroides vulgatus ATCC 8482
Candida albicans ATCC 10231
Escherichia coli ATCC 25922
Micrococcus luteus ATCC 9341
Staphylococcus aureus ATCC 25923
Staphylococcus epidermidis ATCC 12228
Streptococcus pneumonia ATCC 6305
Streptococcus pyogenes ATCC 19615

Result
Growth
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Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.