(AB) UVM Mod, 2 Kilograms

UVM Modified Listeria Enrichment Broth is a modified formula developed by Donnelly and Baigent 1 of the University of Vermont. Nalidixic acid (20 mg/L) inhibits Gram negative microorganisms and acriflavine inhibits Gram positive bacteria. Esculin is utilized by Listeria monocytogenes. Broth medium used for the selective isolation and enrichment of Listeria monocytogenes. (Alpha Biosciences # U21-103-2Kg)

Use: Broth medium used for the selective isolation and enrichment of Listeria monocytogenes.

Description: UVM Modified Listeria Enrichment Broth is a modified formula developed by Donnelly and Baigent 1 of the University of Vermont. Nalidixic acid (20 mg/L) inhibits Gram negative microorganisms and acriflavine inhibits Gram positive bacteria. Esculin is utilized by Listeria monocytogenes.

Formula* per Liter:

• Casein Digest Peptone ................. 5.0g
• Peptic Digest of Animal Tissue ... 5.0g
• Yeast Extract ................................... 5.0g
• Sodium Chloride ......................... 20.0g
• Beef Extract ..................................... 5.0g
• Disodium Phosphate .................... 9.6g
• Monopotassium Phosphate .........1.35g
• Esculin ............................................ 1.0g
• Acriflavine, HCl........................... 0.012g
• Naladixic Acid ........................... 0.020g

Final pH: 7.2 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Mix 52 grams of the medium in a Liter of purified water until evenly dispersed. Heat with repeated stirring to dissolve completely. Distribute and autoclave at 121.0°C for 15 minutes

Quality Control Specifications:

1. The powder is homogeneous, free flowing and light beige.
2. Visually the prepared medium is greenish- yellow to yellow with faint bluish-green ring at the surfacf and very slightly opalescent.
3. Expected cultural response after 24-48 hours at 35.0°C.

Organism                                                                 Result

Escherichia coli ATCC 25922                                 Inhibited

Listeria monocytogenes ATCC 7644                       Growth

Staphylococcus aureus ATCC 25923                     Inhibited

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light beige color.