(AB) Yeast Nitrogen Base 500G

Yeast Nitrogen Base contains all essential nutrients and vitamins necessary for the cultivation of yeasts except a source of carbohydrate. Yeast Nitrogen Base is used for classifying yeasts based on carbon assimilation. (Alpha Biosciences # Y25-104-500gm)

Use: Yeast Nitrogen Base is used for classifying yeasts based on carbon assimilation.

Description: Yeast Nitrogen Base contains all essential nutrients and vitamins necessary for the cultivation of yeasts except a source of carbohydrate.

Formula* per Liter:

• Ammonium Sulfate ...................... 5.0g
• L-Histidine Monohydrochloride ....... 10mg
• LD-Methionine ........................... 20mg
• LD-Tryptophan ............................... 0.02mg
• Biotin ...................................... 0.002mg
• Calcium Pantothenate ..................... 0.4mg
• Folic Acid .............................. 0.002mg
• Inositol .................................................. 2mg
• Niacin ......................................... 0.4mg
• p-Aminobenzoic Acid ..................... 0.2mg
• Pyridoxine Hyrochloride ........... 0.4mg
• Riboflavin ......................................... 0.2mg
• Thiamine Hydrochloride ........... 0.4mg
• Boric Acid ........................................ 0.5mg
• Copper Sulfate ....................... 0.04mg
• Potassium Iodide ............................ 0.1mg
• Ferric Chloride .......................... 0.2mg
• Manganese Sulfate ......................... 0.4mg
• Sodium Molybdate ................... 0.2mg
• Zinc Sulfate ...................................... 0.4mg
• Monopotassium Phosphate ....... 1.0g
• Magnesium Sulfate ............................ 0.5g
• Sodium Chloride .......................... 0.1g
• Calcium Chloride ............................... 0.1g

Final pH: 5.4 ± 0.2 at 25.0°C

* Grams per liter may be adj. or formula supplemented to obtain desired performance

Preparation: Prepare a 10X solution by dissolving 6.7 grams of Yeast Nitrogen Base and 5 grams of Dextrose or equivalent amount of other carbohydrate in 100ml distilled or deionized water. Warm slightly to dissolve. Mix well. DO NOT BRING TO A BOIL. DO NOT AUTOCLAVE. Filter-sterilize the solution. Store at 2-8°C. Prepare the final medium by aseptically pipetting 0.5ml of the 10X solution into 4.5ml of sterile distilled water. Mix the solution thoroughly by shaking before inoculation.

Quality Control Specifications:

1. The powder is homogeneous, free flowing and off-white.
2. Visually the prepared medium is clear and almost colorless.
3. Expected cultural response after 2-5 days at 25.0°C.

Organism                                                           Result with Dextrose

Candida albicans ATCC 10231                              Good Growth

Kloeckera apiculata ATCC 9774                            Good Growth

Saccharomyces pastorianus ATCC 9080                Good Growth

Storage: Store the sealed bottle containing the dehydrated medium at 2 to 30.0°C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original off-white color.